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anti il 1α  (R&D Systems)


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    Structured Review

    R&D Systems anti il 1α
    Anti Il 1α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+il+1%CE%B1/Mouse+IL-1+alpha%2FIL-1F1+Antibody/pm41870812-54-6-7
    Average 93 stars, based on 39 article reviews
    anti il 1α - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence
    Article Snippet: The cells were incubated with 2.5 μM H 2 DCFDA (Invitrogen) or 5 μM MitoSOX (Invitrogen) in culture medium for 30 min at 37 °C, and then washed with PBS. .. The cells were stained with anti-IL-1α (membrane form) [3405] (#FAB200F; R&D Systems) together with the pre-stain antibodies described above. .. For cytoplasmic IL-1α, pre-stained cells were fixed using the Cytofix/Cytoperm kit (BD Biosciences) for 20 min at 4 °C, before staining with anti-IL-1α (cytoplasmic forms) [4414] (#IC200F; R&D Systems) in Perm/Wash Buffer (BD Biosciences) for 30 min at room temperature.

    Membrane:

    Article Title: The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence
    Article Snippet: The cells were incubated with 2.5 μM H 2 DCFDA (Invitrogen) or 5 μM MitoSOX (Invitrogen) in culture medium for 30 min at 37 °C, and then washed with PBS. .. The cells were stained with anti-IL-1α (membrane form) [3405] (#FAB200F; R&D Systems) together with the pre-stain antibodies described above. .. For cytoplasmic IL-1α, pre-stained cells were fixed using the Cytofix/Cytoperm kit (BD Biosciences) for 20 min at 4 °C, before staining with anti-IL-1α (cytoplasmic forms) [4414] (#IC200F; R&D Systems) in Perm/Wash Buffer (BD Biosciences) for 30 min at room temperature.

    Incubation:

    Article Title: Pseudomonas aeruginosa Induced Airway Epithelial Injury Drives Fibroblast Activation: A Mechanism in Chronic Lung Allograft Dysfunction
    Article Snippet: .. Samples were blocked in 1% bovine serum albumin (BSA) and incubated overnight at 4°C with anti‐IL‐1α (AF‐200‐NA, R&D) or an IgG1 control antibody (both 10 μg/mL, 1% BSA). .. Slides were washed and incubated with a fluorescein isothiocyanate–conjugated anti‐goat secondary antibody (F7367, Sigma) for 2 h at room temperature.

    Control:

    Article Title: Pseudomonas aeruginosa Induced Airway Epithelial Injury Drives Fibroblast Activation: A Mechanism in Chronic Lung Allograft Dysfunction
    Article Snippet: .. Samples were blocked in 1% bovine serum albumin (BSA) and incubated overnight at 4°C with anti‐IL‐1α (AF‐200‐NA, R&D) or an IgG1 control antibody (both 10 μg/mL, 1% BSA). .. Slides were washed and incubated with a fluorescein isothiocyanate–conjugated anti‐goat secondary antibody (F7367, Sigma) for 2 h at room temperature.

    Blocking Assay:

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer
    Article Snippet: .. The following blocking antibodies were used: anti-IL-1α (R&D systems, Cat. AF-400-NA; RRID: AB_354473), anti-IL-6 (R&D systems, Cat. MAB406; RRID: AB_2233899), anti-CXCL1 (R&D systems, Cat. MAB453; RRID: AB_2087696), and anti-TNF-α (R&D systems, Cat. MAB4101; RRID: AB_2240643). .. Recombinant IL-6 protein was purchased from R&D systems (Cat. 406-ML).

    Article Title: Targeting senescence-like tumor-associated macrophages sensitizes chemotherapy in triple-negative breast cancer.
    Article Snippet: .. The following blocking antibodies were used: anti-IL-1α (R&D systems, Cat. AF-400-NA; RRID: AB_354473), anti-IL-6 (R&D systems, Cat. MAB406; RRID: AB_2233899), anti-CXCL1 (R&D systems, Cat. MAB453; RRID: AB_2087696), and anti-TNF-α (R&D systems, Cat. MAB4101; RRID: AB_2240643). .. Recombinant IL-6 protein was purchased from R&D systems (Cat. 406-ML).



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    Ultrashort wave therapy improves histopathology and inhibits apoptosis in sinus mucosal samples. (A) Hematoxylin and eosin staining revealed the pathological changes in sinus mucosal samples. (B and C) Apoptosis in sinus mucosa across different groups was assessed using TUNEL assay. Scale bars, 50 and 20 µm. ** P<0.01 and **** P<0.0001. N=6. CRS, chronic rhinosinusitis; ns, no significance.
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    Image Search Results


    a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

    Journal: bioRxiv

    Article Title: Microbiome-derived hydroxyphenyl propanoates enhance antitumour immunity by potentiating gasdermin D activity in tumour-associated myeloid cells

    doi: 10.64898/2026.04.23.720410

    Figure Lengend Snippet: a, representation of the proteins with denaturing curves significantly altered (red) or unaltered (black) by 3,2-HPP treatment of THP1-Dual™ cells, using thermal proteome profiling (two combined experiments, proteomic coverage: 4301, NPARC test); b, protein-protein interaction network of proteins with denaturation curves altered by 3,2-HPP treatment (red) and the transcriptional regulators of 3, 2-HPP impacted genes in M3-9-M tumours (yellow); c, impact of 3,2-HPP treatment of THP1-Dual™ cells on the denaturation curve of GSDMD (two-combined experiments, NPARC test); d, NF-κB induction in THP1-Dual™ reporter cells treated with conditioned media harvested from WT or Gsdmd- KO THP1 cells treated with LPS ± HPP metabolites ± IL-1α and −1β neutralizing antibody (three combined experiments, one-way ANOVA with Bonferroni multiple comparison test); e-h, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT or Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); i-l, [secreted IL-1α and −1β] in conditioned media harvested from THP-1 WT vs. Gsdmd -KO cells, or human PBMCs, cultured for 16 hours in LPS and NG ± HPPs (two combined experiments, one-way ANOVA with Bonferroni multiple comparison test); m, western blot of THP-1 cells treated with LPS ± NG ± HPPs (representative of two experiments).

    Article Snippet: To neutralize IL-1 receptor signalling, the following antibodies were used: anti-human IL-1α (1 μg/mL; clone 7D4; mabg-hil1a-3; InvivoGen), anti-human IL-1β (1 μg/mL; clone 4H5; mabg-hil1b-3; InvivoGen) and IgG1 isotype control (1 μg/mL; clone T8E5; mabg1-ctrlm; InvivoGen).

    Techniques: Comparison, Cell Culture, Western Blot

    Ultrashort wave therapy improves histopathology and inhibits apoptosis in sinus mucosal samples. (A) Hematoxylin and eosin staining revealed the pathological changes in sinus mucosal samples. (B and C) Apoptosis in sinus mucosa across different groups was assessed using TUNEL assay. Scale bars, 50 and 20 µm. ** P<0.01 and **** P<0.0001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

    Journal: Biomedical Reports

    Article Title: Potential effect and mechanism of ultrashort wave therapy in mice with chronic rhinosinusitis

    doi: 10.3892/br.2026.2109

    Figure Lengend Snippet: Ultrashort wave therapy improves histopathology and inhibits apoptosis in sinus mucosal samples. (A) Hematoxylin and eosin staining revealed the pathological changes in sinus mucosal samples. (B and C) Apoptosis in sinus mucosa across different groups was assessed using TUNEL assay. Scale bars, 50 and 20 µm. ** P<0.01 and **** P<0.0001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

    Article Snippet: The sections were then blocked with 5% BSA (cat. no. ST023; Beyotime Institute of Biotechnology) at room temperature for 1 h. Next, the sections were incubated with primary antibodies against IFN-γ (cat. no. 15365-1-AP), IL-1α (cat. no. 83644-1-RR), TNF-α (cat. no. 17590-1-AP), and IL-10 (cat. no. 60269-1-Ig) (all diluted 1:50; Proteintech Group, Inc.).

    Techniques: Histopathology, Staining, TUNEL Assay

    Ultrashort wave therapy inhibits inflammatory responses in the sinus mucosal samples of mice with CRS. (A-E) Immunofluorescence was performed to assess the expression levels of IFN-γ, IL-1α, TNF-α, and IL-10 in sinus mucosal samples of mice with CRS. (F-I) The concentrations of IFN-γ, IL-1α, TNF-α, and IL-10 in nasal lavage fluid of mice with CRS were measured using ELISA. Scale bar, 100 µm. * P<0.05, ** P<0.01 and *** P<0.001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

    Journal: Biomedical Reports

    Article Title: Potential effect and mechanism of ultrashort wave therapy in mice with chronic rhinosinusitis

    doi: 10.3892/br.2026.2109

    Figure Lengend Snippet: Ultrashort wave therapy inhibits inflammatory responses in the sinus mucosal samples of mice with CRS. (A-E) Immunofluorescence was performed to assess the expression levels of IFN-γ, IL-1α, TNF-α, and IL-10 in sinus mucosal samples of mice with CRS. (F-I) The concentrations of IFN-γ, IL-1α, TNF-α, and IL-10 in nasal lavage fluid of mice with CRS were measured using ELISA. Scale bar, 100 µm. * P<0.05, ** P<0.01 and *** P<0.001. N=6. CRS, chronic rhinosinusitis; ns, no significance.

    Article Snippet: The sections were then blocked with 5% BSA (cat. no. ST023; Beyotime Institute of Biotechnology) at room temperature for 1 h. Next, the sections were incubated with primary antibodies against IFN-γ (cat. no. 15365-1-AP), IL-1α (cat. no. 83644-1-RR), TNF-α (cat. no. 17590-1-AP), and IL-10 (cat. no. 60269-1-Ig) (all diluted 1:50; Proteintech Group, Inc.).

    Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay